Composite

Part:BBa_K2957050:Design

Designed by: Ethan Levy   Group: iGEM19_MIT   (2019-10-21)


CMV-2xGal4 (Gal4 repressible promoter), iRFP720


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1630
    Illegal SpeI site found at 92
    Illegal PstI site found at 1091
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 92
    Illegal PstI site found at 1091
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 677
    Illegal XhoI site found at 1013
    Illegal XhoI site found at 1019
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1630
    Illegal SpeI site found at 92
    Illegal PstI site found at 1091
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1630
    Illegal SpeI site found at 92
    Illegal PstI site found at 1091
    Illegal NgoMIV site found at 794
    Illegal AgeI site found at 1636
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used Modular Cloning Golden Gate Type II Assembly, so we had to consider how parts came together. See more on the MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT>.


Source

Parts of this part were ordered from IDT/Twist and then assembled with other parts (from iGEM/parts in our parts collection/from the Weiss lab) into this plasmid.

References